BMC Biotechnology

The rise in nosocomial infections caused by multidrug-resistant bacteria and the ability of bacteria to form biofilms have necessitated the exploration of alternative antimicrobial strategies. In this study, silver nanoparticles (AgNPs) were synthesised using a green method in the presence of curcumin to suppress the biofilm-forming abilities of Methicillin-resistant Staphylococcus aureus and Pse…

Biofloc-based systems provide a sustainable strategy for nitrogen management in intensive aquaculture, but their efficiency depends strongly on carbon availability and microbial community regulation. In this study, we evaluated the combined effects of carbon-to-nitrogen (C: N) ratio regulation and Candida utilis supplementation on nitrogen removal and microbial community dynamics in a biofloc-bas…

Plant-derived exosome-like nanovesicles (pEVs) have emerged as promising natural biomaterials due to their safety profile, high biocompatibility, and rich bioactive cargo. In particular, pEVs have attracted interest because of their roles in intercellular communication. This study aimed to isolate and comprehensively characterize pEVs derived from Equisetum arvense (H-EVs) and Lavandula angustifo…

Lusang (Morus alba var. multicaulis) represents both the inception and paradigm of artificial cultivation and breeding of mulberry (Morus spp.) in China, marking the onset of mulberry domestication. However, the genetic structure underlying this domestication process remains poorly understood, and no reports to date have described the selection signals and their candidate genes, or mRNA–miRNA reg…

This work investigated the capacity of Zingiber officinale extract to synthesize copper oxide nanoparticles. Z. officinale extract is rich in diverse phytochemical constituents, as confirmed by GC–MS analysis. The synthesized CuO NPs were characterized using TEM, FTIR, XRD, SEM, and EDX analyses. CuO NPs exhibited antibacterial efficacy against various bacterial strains. The DPPH methodology was …

Acute primary angle-closure glaucoma (APACG) and chronic primary angle-closure glaucoma (CPACG) exhibit distinct clinical features, yet the molecular mechanisms underlying their differing progression rates remain unclear. This study integrates metabolomics and machine learning to identify subtype-specific metabolic profiles and serum biomarkers for distinguishing APACG from CPACG. A total of 128 …

Matrix stiffness is a key feature of the mechanical microenvironment. Periodontal tissue is a multicomponent structure composed of cementum, periodontal ligament, and alveolar bone, and is essential for the physiological reconstruction and stress of teeth. Simulating the stiffness of periodontal tissue with a biomimetic extracellular matrix is ​​of great significance for regulating cell behavior …

Polydeoxyribonucleotide (PDRN), a DNA polymer derived from salmonid tissue, is widely used in regenerative dermatology but its topical application is limited by poor stability and delivery efficiency. Exosomes, nanoscale vesicles with high cellular uptake and low immunogenicity, offer a promising platform for nucleic acid protection and targeted delivery. In this study, we developed an electropor…

To evaluate the mechanistic synergy of an ononin-modified chitosan/β-glycerophosphate (CS/β-GP) hydrogel loaded with adipose-derived stem cells (ADSCs) in repairing diabetic wounds (DWs). We specifically aimed to determine if ononin could biochemically remodel the hostile diabetic microenvironment to enhance ADSCs survival and potency. Primary rat ADSCs were characterized by flow cytometry. A the…

Antimicrobial peptides are being explored as anticancer agents due to their selectivity toward transformed cells. We evaluated a small peptide from Lacticaseibacillus casei for anti-proliferative effects on A549 lung cancer cells and investigated apoptosis-related mechanisms in vitro. Cell viability was assessed by MTT. Apoptosis-associated morphology was examined by AO/EB staining, DNA fragmenta…

In vitro culture of callus is an effective method for conserving the genetic resources of economically important crops. However, continuous subculturing is a costly and labor-intensive process. Therefore, establishing an efficient in vitro long-term conservation system applicable to various plant species is required. In this study, calli derived from five medicinal plant species, Camellia japonic…

The integrated approach not only valorizes low-cost agro-industrial byproducts but also provides a robust, eco-friendly biocatalyst for wastewater treatment, highlighting a promising circular economy strategy for textile effluent detoxification.

This study presents the electrospinning fabrication of thermoplastic polyurethane (TPU) nanofibrous scaffolds with two fiber architectures; randomly oriented and unidirectionally aligned incorporating olive oil and copper oxide nanoparticles (CuO NPs) to enhance biomedical performance. Both scaffold types exhibited robust antibacterial activity: randomly oriented TPU/CuO mats produced inhibition …

Nattokinase (NK) in recent times has been identified as an important enzyme for its health benefits and its application in cardiovascular diseases (CVD). NK is known for its thrombolytic activity and has been actively used its low cost of production. The research study aims to enhance the Nattokinase production from fermented Brassica oleracea. Seven different fermented and non-fermented food sou…