p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei
Jannatul Firdous Siddique·Mohanasrinivasan Vaithilingam·C. Subathra Devi·G. R. Shree Kumari·Sudarshana Deepa Vijay Kumar
Antimicrobial peptides are being explored as anticancer agents due to their selectivity toward transformed cells. We evaluated a small peptide from Lacticaseibacillus casei for anti-proliferative effects on A549 lung cancer cells and investigated apoptosis-related mechanisms in vitro. Cell viability was assessed by MTT. Apoptosis-associated morphology was examined by AO/EB staining, DNA fragmentation by agarose gel electrophoresis, and gene expression by qRT-PCR (p53, BAX, BCL-2, CASP8, CASP7, C