Bioresources and Bioprocessing

Abstract Tobacco aging is a critical for developing desirable flavor profiles, driven primarily by microbial and enzymatic activities. This study systematically evaluated the effects of Bacillus clausii inoculation (FJ) and B. clausii -cellulase co-treatment (JM) on the surface microbial communities and aroma compounds of Yunyan 87 tobacco leaves during 9 months of aging, with natural aging as th…

Abstract Natural products are rich therapeutic sources, yet their translation into effective medicines remains challenging. Bee venom (BV) contains a diverse repertoire of bioactive molecules, but gentle, scalable methods to enhance its functionality are limited. This study, therefore, investigates a novel, solvent-free, post-extraction infrared (IR) conditioning step to fine-tune BV's compositio…

Abstract The valorization of anaerobic digestates through slow pyrolysis offers a sustainable pathway for agricultural systems. This study assessed the effects of digestate type (swine, cattle, and dairy) and pyrolysis temperature (400, 500, and 600 °C) on biochar properties and evaluated their impact on maize ( Zea mays ) fodder grown under hydroponic-like soilless conditions. Thirty-six treatme…

Abstract Membrane fouling during ultrafiltration/diafiltration (UF/DF) remains a major challenge for extending membrane lifetime in biologics manufacturing. Developing cleaning strategies that combine high efficiency with scalability is essential for robust downstream processing. In this study, different UF/DF membrane cleaning methods were assessed under bench-scale conditions with consideration…

Abstract This study investigates the optimisation of poly(3-hydroxybutyrate) (PHB) production by Mycolicibacterium smegmatis using sugarcane bagasse (SCB) hydrolysate as a low-cost, renewable carbon source. Key fermentation parameters including temperature, pH, agitation, inoculum size and nitrogen supplementation were optimised to enhance biomass growth and PHB accumulation. Under optimised cond…

Abstract Acacia seyal is a medicinal plant rich in bioactive compounds known for their antimicrobial, antioxidant, and anticancer properties. Conventional plant extracts often suffer from poor stability and limited bioavailability, reducing their therapeutic effectiveness. The utilization of chitosan nanoparticles (CS-NPs) offers a promising strategy to enhance biological activity of plant extrac…

The genus Ligustrum (Oleaceae) encompasses woody plants with both medicinal and edible uses, distinguished by a wide range of bioactive compounds, including triterpenoids, phenylethanoid glycosides, flavonoids, and other active constituents. These metabolites demonstrate multi-target pharmacological effects, such as anti-inflammatory, antioxidant, antitumor, and anti-osteoporotic activities. In t…

Allergic rhinitis (AR) is a prevalent inflammatory disorder of the upper respiratory tract, affecting 20-40% of the global population and severely impairing quality of life. Given the limitations and adverse effects associated with conventional pharmacotherapy, naturally derived bioactives with low toxicity are gaining prominence as alternative interventions. In this study, we developed a bioreso…

The yeast Komagataella phaffii is an emerging microbial host for the production of functional recombinant proteins. However, proteolytic degradation during fermentation often compromises product yield and stability, posing a major hurdle for industrial-scale applications. This study presents a strategy to enhance the production of recombinant humanized type I collagen (rhColI) by engineering a ho…

Agricultural residues like sugarcane bagasse and rice straw are rich in cellulose and xylan. Their efficient conversion into (oligo)saccharides and value-added products requires microbial cellulases and xylanases, but low enzyme yields and high production costs hinder industrial application. This study isolated Penicillium oxalicum UNN1, a high-xylanase-producing strain with an initial activity o…

Climate change and environmental pollution are among the most pressing global challenges today, with water pollution standing out as a particularly critical issue. Industrial wastewater discharge, especially from distilleries, significantly contributes to the degradation of aquatic and terrestrial ecosystems. Molasses-based distilleries are major perpetrators, producing vast quantities of dark br…

The aqueous phase (AP) generated during biomass pyrolysis is often considered a waste product due to its dilute and toxic nature, making it difficult to upgrade. This study explores the potential of using AP as a laccase inducer for the white-rot fungus, Pleorotus ostreatus, and as a mediator in laccase-catalyzed reactions. As an inducer, AP increased laccase production from P. ostreatus to 570 U…

Gut microbiota regulation is a key strategy for treating metabolic dysfunction-associated fatty liver disease (MAFLD). Arbutin (ARB) is a natural hydroquinone active agent with anti-inflammatory and antioxidant effects, as well as regulatory effects on the gut microbiota. However, its therapeutic effect on MAFLD and the responsible mechanisms remain unclear. This study explored the therapeutic ef…

The enzymatic esterification of 5-hydroxymethylfurfural (HMF) with long-chain fatty acids offers a sustainable route for producing biolubricants and other high-value chemicals. This work evaluates the synthesis of 5-hidroxymethylfurfural stearate catalyzed by immobilized lipases in both batch and continuous packed-bed bioreactors, combining molecular dynamics (MD) simulations with experimental va…

2,3-Butanediol (2,3-BDO) is a versatile platform chemical with diverse applications in cosmetics, pharmaceuticals, agricultural and food manufacturing. Among its stereoisomers, optically pure (meso)-2,3-BDO is particularly valuable; however, achieving high titers with stereoselectivity remains challenging in conventional hosts due to byproduct formation, low tolerance, and plasmid instability. In…

Via efficient, scalable, and climate resilient processes, seaweed biorefineries can advance cleaner production, delivering nutrient-rich ingredients with relatively lower land and freshwater requirements. Here, a land-based Ulva ohnoi platform that integrates optimized outdoor tank cultivation, pH-shift protein extraction with dual-product valorization, scale-aware techno-economic analysis (TEA),…

As the terminal management for evaluating the engineering effectiveness of antibiotics production and utilization, the toxic effects of moxifloxacin (MOX) and trace concentration of Cu²⁺ (MOX-Cu) on Caenorhabditis elegans (C. elegans) were investigated at physiological, biochemical, and molecular level. Although the stimulate effects were observed after prolonged exposure (72 h) to MOX…

Wine production generates significant quantities of by-products each year, among which surplus grape must is notable for its high content of fermentable sugars, organic acids, and polyphenols. As wine consumption declines and grape must surpluses grow, identifying sustainable valorization strategies becomes increasingly critical. This study investigates for the first time the potential use of red…