Journal of Assisted Reproduction and Genetics

Abstract Purpose Accurate assessment of oocyte developmental competence remains challenging in IVF, as morphology alone offers limited insight into intrinsic oocyte quality. Emerging evidence indicates that oocyte competence reflects coordinated regulation of the follicular microenvironment rather than a single biomarker. This study aimed to evaluate a multidimensional follicular marker panel int…

Abstract Background Abnormal pronuclear formation, including five pronuclei (5PN), is generally considered indicative of abnormal fertilization (e.g., polyspermy or failure of polar body extrusion) and leads to routine embryo discard in many IVF programs. However, emerging evidence suggests that a minority of embryos with atypical pronuclear patterns may develop into a blastocyst and present dipl…

Abstract Purpose In vitro -produced embryos exhibit reduced developmental competence compared to their in vivo -derived counterparts due to the inability to accurately replicate the complex milieu of the maternal reproductive tract. A key aspect in vivo is the confinement of embryos in small volumes of oviductal and uterine fluids, where maternal extracellular vesicles (EVs) deliver their cargos …

Abstract This review analyzes strategies to prevent or reduce the transmission of diseases caused by pathogenic variants in mitochondrial DNA (mtDNA). Among these, we will focus on prenatal screening, preimplantation genetic testing, gene-editing techniques, other molecular tools, and selected heterologous mitochondrial replacement techniques (MRTs), explaining their status and the uncertainties …

There were significantly lower odds of obtaining a usable embryo when testing for variants in genes in patients aged < 35 and 35-37. No significant difference was seen among patients aged 38-40 or 41-42. These findings provide quantifiable data to counsel patients considering PGT-M testing for variants in two genes.

In ART media, hypoxic oxygen equilibration is a gradual process while reoxygenation is rapid. Oil viscosity significantly influences oxygen equilibration dynamics, with light oil permitting faster equilibration and reoxygenation. These findings underscore the importance of minimizing atmospheric exposure during routine handling and highlight the role of overlay oil in reducing transient oxygen fl…

The decellularization protocol effectively removed cellular content and preserved ECM structure and ultrastructure. 3D culture system combined with supplementation of 0.02 µM RLNP supported follicular viability and ultrastructural preservation and was associated with transcriptional changes in antioxidant-related genes.