Decellularized ovarian bioscaffolds and resveratrol-loaded polymeric nanoparticles support in vitro viability and ultrastructural preservation of bovine preantral follicles
Francisco C. Costa·José Roberto Viana Silva·Miguel Fernandes de Lima Neto·Mariana Aragão Matos Donato·Venância Antônia Nunes Azevedo·Alice Vitória Frota Reis·A.A. Silva·Regislane Pinto Ribeiro·Bianca Régia Silva·Ernando Igo Teixeira de Assis·Josimar Oliveira Eloy·Danisvânia Ripardo Nascimento
The decellularization protocol effectively removed cellular content and preserved ECM structure and ultrastructure. 3D culture system combined with supplementation of 0.02 µM RLNP supported follicular viability and ultrastructural preservation and was associated with transcriptional changes in antioxidant-related genes.