Human breastmilk is a rich source of beneficial microbiota and bioactive compounds, yet its potential as a reservoir of psychobiotics remains underexplored. In this study, breastmilk samples were aseptically collected from healthy lactating mothers and cultured using MRS media. A total of 188 bacterial isolates were screened for gamma-aminobutyric acid (GABA) production using monosodium glutamate (MSG) as a substrate, with quantification by High-Performance Liquid Chromatography (HPLC). Several