Phosphoramidite Monomers as Oligonucleotide Molecular Building Blocks: Structural Types, Functional Roles, and Labeling Strategies

In essence, “monomer” and “molecular building block” are two ways of describing the same class of substances in oligonucleotide chemistry. Both refer to the preassembled units used for chemical synthesis of oligonucleotide chains, namely phosphoramidite monomers. The term “molecular building block” is more common in medicinal chemistry and synthetic biology because it emphasizes the modular, engineerable nature of these compounds. In oligonucleotide drug development, phosphoramidite monomers...