Molecules

The development of alternative fat systems for confectionery glazes requires precise control of melting behavior, solid fat content, and rheological performance. In this study, binary fat systems based on fully hydrogenated rapeseed oil (FHRSO) and refined sea buckthorn oil (RSBO) were developed and modified by chemical interesterification for application in non-tempered confectionery glazes. Int…

Intestinal dysfunction and parenteral nutrition (PN) can trigger a spectrum of liver disorders collectively referred to as intestinal failure-associated liver disease (IFALD), for which therapeutic options remain limited. In the present study, we investigated the modulatory effects of the bioactive xanthophyll carotenoid lutein in an in vitro IFALD model utilizing human THLE-2 hepatocytes exposed…

Bietti crystalline dystrophy (BCD) is a hereditary retinal disease caused by loss-of-function mutations in the CYP4V2 gene. Gene replacement therapy using rAAV-hCYP4V2 represents a promising therapeutic strategy, requiring robust bioassays for product quality control. This study developed and validated a sensitive LC-MS/MS method for quantifying CYP4V2 enzyme activity. Lysates from HeLa-AAVR cell…

The synthesis of nitrogen-containing heterocycles remains a subject of significant interest due to their applications in medicinal chemistry and materials science. This paper describes the preparation of imidazo[1,2-a]pyridine using a catalytic system consisting of the deep eutectic solvent (DES) choline chloride (ChCl)–tartaric acid (1:2) and I2 by reaction between 2-aminopyridines and chalcones…

Bread enriched with broccoli stalk powder is proposed as a newly formulated product with potential health benefits. Wheat flour in the bread recipe was enriched with powder obtained from freeze-drying broccoli stalks, a valuable by-product of vegetable processing. The effects of broccoli stalk powder (BSP) supplementation on the physicochemical and sensory properties of bread, as well as its bioa…

Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for molecular analysis, yet the recognition of mixed spectra remains challenging because severe peak overlap makes mixture-specific data expensive to acquire and difficult to cover exhaustively. Current machine-learning approaches often rely on labeled mixture datasets, synthetic mixed spectra, or prior component-matching schemes, maki…

Anemia is a major global public health problem and is most commonly associated with iron deficiency; however, deficiencies in other micronutrients, including vitamin B12, may also contribute to its development. Increasing evidence suggests that Helicobacter pylori infection may influence the occurrence of anemia through several mechanisms related to alterations in the gastric environment. Chronic…

Chalcones, a subclass of flavonoid-derived phenolic compounds, have demonstrated promising anthelmintic activity against parasitic nematodes. This study aimed to obtain insights into the biological effects a cis/trans mixture of chalcone and its geometric isomer, trans-chalcone, using RNA sequencing in the model organism Caenorhabditis elegans. Fourth-stage larvae (L4) were exposed to cis/trans-c…

The field of nanoparticle-based biotechnology has undergone substantial advancement, characterized by progress in targeted drug delivery systems, the development of innovative diagnostic and imaging platforms, the expanded adoption of environmentally sustainable (“green”) synthesis approaches, and an increasing emphasis on the integration of emerging technologies such as artificial intelligence a…

This study focuses on Lacto-N-neotetraose (LNnT), a core component of human milk oligosaccharides. Although LNnT has been demonstrated to promote early intestinal development and maintain gut homeostasis, its protective mechanism against D-galactose-induced intestinal injury and associated cognitive impairment remains unclear. This investigation systematically examined the protective effects and …

Boron neutron capture therapy (BNCT) is a binary targeted radiotherapy that uses boron agents to treat refractory malignancies. This study developed a novel boronophenylalanine (BPA)-loaded liposome doped with o-carborane (CB) for BNCT. We applied response surface methodology (RSM) to identify factors affecting BPA loading and optimized encapsulation efficiency (EE) to minimize BPA loss. In in vi…

Wood is a material frequently used in the production of alcoholic beverages. Oak barrels have been used to store wines, beers, and spirits for generations. Nowadays, beverages are increasingly matured in the presence of staves and chips from various wood species. The aim of this article was to describe the impact of different wood species and their thermal processing conditions on the quality of …

This review summarizes current knowledge on naturally occurring pristimerin-pristimerin triterpenoid dimers, a rare and structurally diverse class of secondary metabolites reported mainly from Celastraceae species. Known dimers are compiled with emphasis on botanical sources and key architectural features, including the variety of interunit linkages, regio- and stereochemical diversity, and disti…

Metabolic reprogramming is a core hallmark of malignancy, enabling tumor cells to sustain rapid proliferation, evade immune elimination, and develop resistance to therapy. Although a wide range of plant-derived phytochemicals exhibit anticancer activity with comparatively low toxicity, their capacity to disrupt specific metabolic dependencies exploited by tumors has not been comprehensively synth…

Traditional polymer-dispersed liquid crystal (PDLC) faces limitations in smart dimming applications due to high driving voltage and poor high-temperature stability. In this study, a high-birefringence liquid crystal (QYPDLC-901) was used to prepare PDLC films with liquid crystal contents ranging from 72 wt% to 80 wt%, achieved through synergistic regulation of a low-functional acrylic polymer sys…

Diabetic kidney disease (DKD) affects approximately 40% of patients with diabetes mellitus and remains a leading cause of end-stage renal disease worldwide. Early diagnosis and identification of therapeutic targets are critical for improving patient outcomes, yet reliable biomarkers are lacking. This study integrated transcriptomic data from the Gene Expression Omnibus (GEO) database (GSE96804, G…

Sustainable food processing and zero-waste management of by-products require the search for natural functional ingredients that can be used in finished food products. Edible mushrooms are a rich source of non-volatile bioactive compounds, but their functionality in complex food matrices remains poorly understood. The aim of the study was to compare the profiles of bioactive non-volatile compounds…

Chemotherapy-induced gonadotoxicity severely compromises male fertility, yet effective interventions remain limited. Building on our previous finding that yam protein (YP) modulates the gut-microbiota axis, this study investigated its direct protective role against cyclophosphamide (CTX)-induced testicular injury. Spectral analysis revealed a protein fraction (L-YP) with strong intrinsic fluoresc…

Coffee is a very popular psychoactive beverage with a complex composition. Besides its stimulant effect due to caffeine, it contains several bioactive compounds with antioxidant properties and potent metabolic activity. Its clinical efficacy is fundamentally determined by the bioavailability and metabolic fate of its constituents. The bioactive components of coffee, such as polyphenols, melanoidi…

Annona cherimola is a plant species widely used in Mexican traditional medicine, particularly in the management of diabetes. This study aimed to investigate the antihyperglycemic properties of the petroleum ether extract of A. cherimola leaves (PEEAcL), as well as to evaluate their effects on glycated hemoglobin and toxicity. In addition, the work was directed to determine its potential as an SGL…