Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet alone study. To elucidate these interactions scientists have long used antibody based affinity purification and mass spectrometry approaches. Recently however, scientists have developed newer methods termed proximity-based labeling to better capture these interactions in a vast array of biological...