STRAIGHT-IN Dual: a platform for dual single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells
Richard P. Davis
Nature Biomedical Engineering, Published online: 30 April 2026; doi:10.1038/s41551-026-01677-9 STRAIGHT-IN Dual enables allele-specific integration of two DNA constructs into hiPSCs within 1 week. The system was used to programme hiPSCs into distinct cell types and for tunable expression of different transcription factors within the same cell.