Antimicrobials are widely used in intensive poultry production, particularly in sub-Saharan Africa, promoting the selection and spread of antimicrobial-resistant bacteria. Avian fecal Escherichia coli (AFEC) act as important reservoirs of antimicrobial resistance (AMR) genes, reflecting baseline resistance levels and facilitating dissemination of resistance and virulence determinants within poultry production systems. This study aimed to phenotypically and genotypically characterize cloacal E. c