Myxomycetes (true slime molds) are amoebozoan protists involved in decomposition and nutrient cycling in terrestrial ecosystems. Recent molecular studies have led to major taxonomic revisions in Physaraceae, including the establishment of the genus Nannengaella for highly calcified species previously placed in Physarum. To refine the taxonomy and distribution of Nannengaella in China, we used an integrative approach combining multilocus phylogenetics and morphological examination. A total of 149