Non-viral targeted integration of large DNA in primary human T cells independent of double-stranded DNA breaks
Wei Li
Nature Biomedical Engineering, Published online: 30 April 2026; doi:10.1038/s41551-026-01671-1 PRIME-In couples a prime edited donor template with either single or paired genomic nicks to enable precise integration of even large constructs into human cells, as exemplified by high-efficiency integration of a 3-kb CAR construct in T cells.