A CRISPR-Cas13a–based two-step assay combined with lateral flow strips for rapid detection of Epstein–Barr virus
Haiqing Sun
BackgroundEpstein–Barr virus (EBV) is a widespread oncogenic virus associated with multiple human malignancies. Accurate detection of EBV DNA is essential for disease screening and monitoring; however, commonly used methods such as quantitative PCR require specialized instruments and laboratory infrastructure, limiting their applicability in resource-limited settings.MethodsAn isothermal nucleic acid detection method for EBV DNA was developed by combining recombinase-aided amplification with CRI