Integrated cryogenic electron microscopy (cryoEM) and cryogenic electron tomography (cryoET) approach, advanced through NSF’s STROBE, a National Science Foundation Science and Technology Center, enables unparalleled resolution for complex cellular structures like the flagellar doublet microtubules (DMT) of Trypanosoma brucei. This parasite causes African sleeping sickness, affecting millions. The flagellum is essential for motility, infectivity, and . . .