Exploring Applications of the Bioluminescent HiBiT Tag
Guest Blogger
Studying proteins in their natural context is one of the biggest challenges in biology. From tumor suppressors to growth factors, some of the most clinically-relevant proteins are also the hardest to study. One common strategy is protein overexpression — boosting levels so interactions aren't missed. But this often yields results that don't reflect true biology. Fluorescent fusions utilizing bulky tags can disrupt normal cell biology and make it hard to capture true protein behavior....