Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy

Nature Communications, Published online: 17 August 2026; doi:10.1038/s41467-026-76703-0 Understanding molecular orientation is essential for unveiling biological structure and function. Here the authors develop a robust, high-fidelity framework (eGRL) for reconstructing complete 3D density and 3D orientation distributions of fluorescent molecules with polarized fluorescence microscopy.