Gastrodin alleviates cardiomyocyte senescence by regulating autophagy through the AMPK/mTOR/4EBP1 pathway

ObjectiveTo investigate whether Gastrodin (Gas) attenuates doxorubicin (Dox)-induced cardiomyocyte senescence and apoptosis by regulating the AMPK/mTOR/4EBP1 signaling pathway and autophagy-related processes.MethodsA Dox-induced senescence model was established in H9c2 cardiomyocytes, and different concentrations of Gas (0.5 mM and 1 mM) were used for intervention. Senescence phenotypes were evaluated using SA-β-gal staining and senescence-associated proteins (P16, P21, and P53). Furthermore, th