High-throughput Raman-activated cell sorting of microalgal genome-wide edited library revealed a regulatory pathway for carotenoid synthesis

Nature Communications, Published online: 12 June 2026; doi:10.1038/s41467-026-74304-5 Functional genomics in algae are limited by efficient methods that connect genotype and metabolic phenotype at single-cell resolution. Here the authors introduce an approach based on a genome-wide mutant library and a Raman Cytometer, discovering a carotenoid synthesis regulating pathway.