Blog2/24/2026

Binding constants on a microplate reader | BMG LABTECH

Dr Barry Whyte
Introduction This blog is about binding constants (association, dissociation or equilibrium constants), how to measure them, and the crucial role they play in understanding molecular interactions. By definition, a binding constant quantifies the strength of the interaction between two molecules, such as a ligand and its binding partner, under equilibrium conditions. Information on binding constants is important for understanding diverse areas of research where a ligand interacts with a biomolecu