IntroductionViruses in water bodies pose potential threats to human health and ecological environments. Therefore, exploring viral diversity in human-associated lakes and domestic wastewater is crucial.MethodsIn this study, we performed comparative viromic analysis of lake and domestic wastewater samples collected from the same water system in Wenzhou, China.ResultsA total of 13,708,121 sequencing reads were classified into 30 viral families, including viruses associated with animals, plants, an