A transgenic rotifer-based RNA interference approach for antiviral protection against Covert mortality nodavirus in shrimp aquaculture

Viral diseases inflict severe economic losses on global aquaculture, driving the urgent need for sustainable and effective disease management strategies. In this study, we developed a feeding-based RNA interference (RNAi) strategy, utilizing the rotifer Brachionus plicatilis as a live delivery vehicle for short hairpin RNA (shRNA) against Covert mortality nodavirus (CMNV) to control covert mortality disease (VCMD) in Penaeus vannamei. A piggyBac-mediated transgenic platform was established in B.