BackgroundThis paper addresses a critical challenge in developing practical EEG-based brain-computer interfaces (BCIs): enhancing cross-subject generalization by mitigating individual differences in brain signals. How can we effectively leverage data from existing subjects to improve performance for a new user with minimal subject-specific calibration?MethodsWe systematically compare and optimize three prominent data alignment techniques, Riemannian Procrustes Analysis (RPA), Euclidean Alignment (EA), and Correlation Alignment (CORAL), designed to transform EEG data from multiple source subjects and a target subject into a common representation space, mitigating variability.EvaluationWe employed leave-one-subject-out cross-validation (LOSO-CV) framework on EEG-based attention decoding data to empirically evaluate the effectiveness of each alignment method compared to a baseline condition with no alignment. Key parameters, specifically the regularization parameter α for EA, were optimized to maximize cross-subject transfer performance.ResultsThe study demonstrates that alignment methods improve classification accuracy compared to the baseline. Notably, EA evaluated at α = 100 the scaling value at which the largest fraction of subjects attained their best accuracy in our parameter sweep yielded the largest mean improvement, increasing classification accuracy by 3.44% over the no alignment baseline (paired t(17)≈2.48, p≈0.024; Cohen's dz≈0.59; 95% confidence interval for the mean improvement [0.52%, 6.36%]). Because this α value was identified from the same sweep that produced the per-subject accuracies, this estimate together with the per-subject “best-parameter” results should be interpreted as an oracle sensitivity-analysis upper bound on subject-specific tuning rather than as a leakage-free LOSO estimate. While optimized EA showed the best mean performance, the analysis also demonstrated subject-specific differences in the most ideal alignment strategy.ConclusionThis comparison framework quantifies the benefits of different alignment approaches and highlights the valuable contribution of parameter optimization, particularly for EA.SignificanceThese results indicate the potential of optimized alignment techniques, EA in particular, to significantly enhance cross-subject transfer learning in EEG-based BCIs. This has practical ramifications for methodology selection and tuning, and maps a path toward more robust and generalizable BCI systems requiring less subject-specific calibration for real-world applications.