Addgene6/17/2025

Plasmids 101: Expressing shRNAs for RNA Interference

Alyssa Shepard
An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most importantly, effective. In the research world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred method for many labs. However, RNA interference (RNAi) remains steadfast in the background, a handy “oldie” as you might say. RNA interference (RNAi) is, as the name might...