BackgroundZedoary Turmeric Oil (ZTO) is an active extract derived from the traditional Chinese medicine Ezhu, which is conventionally used to promote blood circulation, remove blood stasis, and relieve pain. Nevertheless, the therapeutic potential and underlying molecular mechanism of ZTO against tumor brain metastasis (BrM) remain poorly elucidated.MethodsThe anti-BrM efficacy of ZTO was firstly assessed through S100A14-overexpress tuomor cells intracarotid injection BrM mice model. Concurrentl