Brieflow: an integrated computational pipeline for high-throughput analysis of optical pooled screening data

Nature Communications, Published online: 30 May 2026; doi:10.1038/s41467-026-73643-7 Optical pooled screening links genetic perturbations to cellular phenotypes but lacks standardized computational analysis tools. Here, authors present Brieflow, an integrated pipeline for end-to-end analysis of optical pooled screening data with automated biological interpretation.