In situ cryo-ET defines the ultrastructure of ER exit sites in human cells
Giulia Zanetti
Nature Cell Biology, Published online: 20 May 2026; doi:10.1038/s41556-026-01964-2 Downes et al. use in situ cryo-electron tomography and super-resolution fluorescence microscopy to define ER exit site architecture in unperturbed human cells. They visualize ribosome-exclusion zones enriched with COPII- and COPI-coated vesicles.