Antibiotic resistance poses one of the most pressing threats to global public health, underscoring the urgent need for novel antimicrobial agents. Mushroom-derived polysaccharides have attracted considerable attention due to their structural diversity, low toxicity and various pharmacological activities. This review provides a comprehensive overview of the antiviral, antibacterial, and antifungal activities of polysaccharides isolated from edible and medicinal mushrooms against a range of pathog