Toward precision detection of pyrazinamide resistance: critical concentration assessment and rapid molecular method validation
Xiaowei Dai
This study evaluated broth microdilution (BMD) and fluorescence PCR melting curve analysis (MeltPro MTB/PZA, targeting pncA mutations) for detecting pyrazinamide (PZA) resistance-associated mutations in rifampicin-resistant tuberculosis (RR-TB). PZA susceptibility was assessed in RR-TB isolates from patients at TB prevention and control institutions and at designated hospitals in Beijing. Whole-genome sequencing (WGS) was used as a genotypic comparator to assess the agreement of BMD at critical