IntroductionAlcoholic fatty liver disease (AFLD) is one of the most common alcohol-related liver disorders and is characterized by hepatic lipid accumulation, oxidative stress, and hepatocyte injury. Melatonin (MT), a potent antioxidant and free-radical scavenger, has been reported to exert hepatoprotective effects; however, its protective mechanisms against chronic alcohol-induced AFLD remain incompletely understood.MethodsA mouse model of AFLD was established by chronic alcohol administration