Single-molecule kinetic exploration of functional sub-states in an evolving phosphotriesterase
Nobuhiko Tokuriki
Nature Communications, Published online: 02 May 2026; doi:10.1038/s41467-026-72534-1 This study uses single-molecule approaches to detect changes in functional sub-states during enzyme evolution and highlights that coordinated shifts in conformational heterogeneity, and the underlying functional sub-states are key to enzyme function.