Nature Communications, Published online: 10 June 2026; doi:10.1038/s41467-026-74272-w Single cell RNA sequencing expanded the possibilities of capturing immune repertoires in lymphocytes but assembling long sequences from the individual reads, especially in naive and memory B cells with low expression of immunoglobulin transcripts is challenging. Here the authors present a computational tool to assemble and annotate paired heavy- and light-chain B-cell receptor sequences which allows distinction