What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
Ashley Waldron
You have just purified your plasmid and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure your sample really is?
To assess the concentration and purity of nucleic acids, such as plasmids, the go-to method is to use spectrophotometry. Microvolume spectrophotometers make it quick and easy to measure sample concentration and purity using only a tiny bit of the sample itself — so easy that this simple procedure is often taken for...