We have fully characterized the high-valent intermediates formed during the catalytic cycle of lactoperoxidase (LPO), a heme-containing enzyme present in milk, saliva, tears and airways, playing an important role in mammalian antimicrobial defense. Our combined approach of multifrequency (9–285 GHz) Electron Para magnetic Resonance (EPR) and resonance Raman (rR) spectroscopies allowed the identification of Tyr21 and Tyr193 as radical sites, having a concerted role with the heme iron and resulti
Lactoperoxidase: Identification of the Two Tyr Radical Sites for the Stabilization of the pH-Dependent [Fe(IV) = O Tyr•] Catalytic Intermediates by Resonance Raman and Multifrequency Electron Paramagnetic Resonance Spectroscopies
Piotr Mak et al.