IntroductionPumpkin production in Guangxi, China, is increasingly affected by complex viral diseases, but the regional viral spectrum and mixed-infection structure in symptomatic pumpkin plants remain insufficiently characterized.MethodsWe analyzed 289 symptomatic pumpkin samples collected across Guangxi during 2022-2025 using small-RNA sequencing for virus discovery and targeted PCR/RT-PCR assays for individual-sample confirmation. Virus prevalence, mixed-infection profiles, regional and host-a
Pervasive mixed infections and recurrent begomovirus co-detection in symptomatic pumpkin plants from Guangxi, China
Wenjun Liu