Nature Communications, Published online: 05 June 2026; doi:10.1038/s41467-026-74020-0 Monitoring structural changes in enzymes is essential for elucidating the mechanisms and dynamics of enzymatic catalysis, however, the transient conformational fluctuations involved in catalytic processes typically occur on microsecond-to-millisecond timescales, posing significant challenges for detection via conventional techniques. Here, the authors investigate the catalytic dynamics of cytochrome P450 1A1 du