Boosting prime editing with engineered non-canonical pegRNAs
Chun-Qing Song
Nature Biomedical Engineering, Published online: 07 April 2026; doi:10.1038/s41551-026-01650-6 Prime editing using non-canonical prime editing guide RNAs, where the reverse transcription template and primer binding site is integrated within the single guide RNA loops rather than attached to the 3′ ends of CRISPR–Cas guide RNAs, increases editing efficiency.