IntroductionThe gut microbiota is a critical modulator of host energy metabolism and immune regulation. Although exercise modulates microbial composition, the temporal dynamics and strain-specific impact of acute high-intensity exercise remains unclear.MethodsThis study investigated dynamic alterations in gut microbiota following acute high-intensity exercise in BALB/c and C57BL/6 mice, focusing on energy and gut health-related genera. Age-matched male mice underwent a 30-min high-intensity trea