Structural basis for dual mechanism of Cas2/3 nuclease inhibition by anti-CRISPR protein AcrIF19
Yongchao Xie
Nature Communications, Published online: 19 May 2026; doi:10.1038/s41467-026-73156-3 CRISPR-Cas systems are prokaryotic immune defenses commonly targeted by phage-encoded anti-CRISPR (Acr) proteins. Here, authors report cryo-EM structures of Cas2/3 and its complex with AcrIF19 from Pectobacterium atrosepticum, and uncover its dual inhibitory mechanism against Cas2/3 function.