Microorganisms

A novel bacterial strain, CR191T, was isolated from maize (Zea mays) rhizosphere soil. The strain is Gram-stain-negative, oxidase-positive, rod-shaped, non-motile, and aerobic. Phylogenetic analysis based on 16S rRNA gene sequences placed CR191T within the genus Dyella, with highest similarity to Dyella flava DHOC52T (98.23%) and Dyella dinghuensis DHOA06T (98.16%). The genomic DNA G+C content wa…

Unusually frequent and soft stools may negatively impact the quality of life. A postbiotic containing heat inactivated Limosilactobacillus fermentum CNCM I-2998 and Lactobacillus delbrueckii subsp. lactis CNCM I-4831 has previously been shown to alleviate gastrointestinal symptoms and diarrhea in patients with clinically diagnosed gastrointestinal disorders. This study evaluated whether this post…

Salinity is a key driver of microbial community structure and function in salt lake ecosystems, yet how it shapes functional genes involved in carbon (C), nitrogen (N), and phosphorus (P) cycling remains poorly understood. We collected metagenomic samples along a natural salinity gradient in Yuncheng Salt Lake and examined how salinity was associated with microbial taxonomic and functional divers…

Arthropod-borne viruses such as Alphaviruses and Flaviviruses are the causative agents of severe human disease, highlighted by fatal encephalitis and neurological sequelae in survivors. The lack of FDA-approved vaccines and therapeutics that can prevent or treat these infections results in a significant global disease burden. An important unmet need to address this capability gap is the need for …

Microbiologically influenced corrosion (MIC) at oral biomaterial interfaces is viewed as an ecological phenomenon, yet how microbial composition and interspecies interactions shape corrosion remains unclear. Here, we investigated whether oral microbial community composition and interspecies interactions contribute to medical 316L stainless steel corrosion. Consortia-enhanced Cr and Fe release and…

Blood cultures are frequently obtained in hospitalized patients with COVID-19, although the diagnostic yield and clinical meaning of culture positivity vary markedly according to case mix, disease severity, and timing of sampling. Omicron-era data remain comparatively limited. We aimed to characterize blood-culture positivity, pathogen distribution, inflammatory correlates, antimicrobial exposure…

Pseudomonas aeruginosa is a nosocomial pathogenic bacterium that causes a wide range of human infectious diseases. The type III secretion system (T3SS) serves as a key virulence determinant underlying the pathogenesis of this bacterium in acute infections. As the master transcriptional activator of T3SS, ExsA binds to target promoter regions and modulates the expression of all currently identifie…

Surveillance of methicillin-resistant Staphylococcus aureus (MRSA) in the environment is crucial for reducing its transmission, particularly in hospitals, nursing homes, and high-contact public areas. Rapid Point-of-Need (PON) tools at healthcare facilities, without involving prolonged bacterial culture in sophisticated laboratories, are needed for environmental surveillance of MRSA. We have deve…

Fresh cheeses are highly perishable dairy products due to their high moisture content, pH, and other intrinsic characteristics that support microbial growth. This scoping review synthesized the available evidence on spoilage-associated bacteria in fresh cow cheeses, the physicochemical and sensory changes associated with spoilage, and the methods for their identification and quantification. The r…

More than six decades of human activity in Antarctica resulted in chronic diesel contamination of soils surrounding research stations. Phytoremediation assisted by the native vascular plant Deschampsia antarctica is one of the few remediation strategies compatible with the Antarctic Treaty System guidelines. Securing suitable root-associated microbial resources is central to this approach. This w…

Understory intercropping may improve soils, but its effects on bacterial communities in Idesia polycarpa plantations remain unclear. Five patterns were established in the Yellow River floodplain: pure I. polycarpa and I. polycarpa intercropped with Astragalus membranaceus, Ipomoea batatas, Salvia rosmarinus, or Arachis hypogaea. Soil properties and bacterial communities were assessed using 16S rR…

Precision nutrigenomics requires a diet–microbiome–host perspective because microorganisms can transform feed substrates, generate bioactive metabolites, compete with pathogens, and modify intestinal and systemic gene regulation. This structured narrative review synthesizes representative controlled feeding trials, transcriptomic and targeted gene-expression studies, microbiome analyses, and comp…

The honeybee (Apis mellifera) gut microbiota serves as a model for host–microbe studies, as newly emerged workers provide a low-complexity, nearly germ-free system. Although multiple inoculation strategies are commonly applied under laboratory conditions, their comparative effects on microbial colonization remain incompletely understood. Here, we systematically compared four commonly used inocula…

Hydrothermal systems contain diverse alkanes derived from biological and geological processes, providing potential carbon and energy sources for microorganisms. However, cultivated thermophilic alkane-utilizing bacteria from hydrothermal environments remain poorly characterized. In this study, a strain L01 was isolated from a deep-sea hydrothermal vent and identified as an alkane-degrading bacter…

Three Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strains, designated LJC-15T, NGA-4T and QZ2-6-4T, were isolated from the rhizosphere soils of Capsicum, Cucurbita and Solanum melongena, respectively, in Yunnan Province, China. Phylogenetic and phylogenomic analyses placed the three strains within the genus Agromyces but distinguished them from recognized species. All ANI and d…

This study investigated the effects of nutritional conditions and exogenous N-acyl-homoserine lactones (AHLs) on the recovery of sodium hypochlorite (NaClO)-induced viable but non-culturable (VBNC) Vibrio parahaemolyticus. Exposure to 40 mg/L NaClO for 20 min resulted in the loss of culturability while viable cells (approximately 1.19 × 102 cells/mL) remained detectable by SYTO9/PI staining, cons…

Although most upper respiratory tract infections (URTIs) have a viral etiology, antibiotics are widely prescribed, thus contributing substantially to antimicrobial resistance. This study investigated the potential beneficial effects of a novel strain, Limosilactobacillus reuteri LMG P-27481, against major URT pathobionts (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Mo…

Aspergillus westerdijkiae, a filamentous fungus commonly isolated from pickled vegetables and high-salt condiments, can cause spoilage and produce nephrotoxic ochratoxin A (OTA) under saline conditions. However, its adaptive mechanisms to salt stress remain unclear. To address this, the pickle-derived strain NDX1 was subjected to 0, 1.0, 1.5, and 2.0 mol/L NaCl treatments. Colony growth was asses…

Plants and their endophytic microbiota form a functional holobiont, wherein host-dependent endophytes (HDEs) are proposed to serve as persistent microbial components associated with host continuity. Using long-term in vitro grapevine plantlets, we investigated HDE redistribution and reassembly across successive agar-based subcultures and during the transition to soil-based cultivation. Results sh…

Currently, there is no effective ELISA detection methods for orf virus (ORFV) based on recombinant proteins and monoclonal antibodies(mAbs). This research aims to express recombinant B2L protein (rB2L) of ORFV and prepare high specificity monoclonal antibodies targeting rB2L. After codon optimization for ORFV B2L gene, the optimized gene fragment was chemically synthesized and ligated into a prok…