Empowered pro-inflammatory features in Ly6C monocytes and altered antigen-presenting capacity in Ly6Chigh monocytes in diabetic mice
Pingping Yang·Hong Wang·Lizhe Sun·Lu Liu·Linghua Fu·Hang Xi·Xianwei Wang·Rihab Bouchareb·Daqing Zhang·Qinghua Wu·Yong Ji·Xiaofeng Yang·Pu Fang
Rationale Inflammatory monocyte (MC) subset polarization is a hallmark of systemic and tissue inflammatory feature in diabetes. The underlying molecular mechanism remains unclear. Methods and results Blood pro-inflammatory Ly6C high and anti-inflammatory Ly6C low MC subsets were isolated from control (C57/BL6), type 1 diabetes mellitus (T1DM), and type 2 diabetes mellitus (T2DM) mice by flow cytometry sorting and subjected to bulk high-throughput RNA-sequencing. Intensive and integrative functio