Bridging continuous and discrete evolution through a controllable, hypermutagenic phage-bacteria system
Julius Fredens
Nature Microbiology, Published online: 01 May 2026; doi:10.1038/s41564-026-02346-y The lytic selection and evolution (LySE) system uses a plasmid-encoded, hypermutagenic T7 DNA polymerase and T7 phagemid-encoded target genes to enable controllable, near-continuous directed evolution of large gene clusters.