ObjectiveThe research is to explore the half effective dose (ED50) and 95% effective dose (ED95) of ciprofol with intraperitoneal injection of 8-week-old C57BL/6J adult mice, which will provide experimental basis for the reasonable dose design of ciprofol in preclinical basic research.MethodsC57BL/6J mice were selected and divided into 8 dose groups (50, 55, 60, 65, 70, 80, 90, 100 mg/kg), with 12 males and 12 females in each group. The ciprofol was administered by intraperitoneal injection, and