Recent advances in rapid multiplex detection of nucleic acid markers using RPA and CRISPR-Cas
Lei Jiang
The integration of recombinase polymerase amplification (RPA) with CRISPR-Cas systems has emerged as a powerful platform for rapid multiplex nucleic acid detection. Compared with quantitative polymerase chain reaction (qPCR) and Next-generation sequencing (NGS), RPA-CRISPR operates isothermally (37 °C–42 °C), requires minimal equipment, and achieves attomolar sensitivity in 20–90 min via collateral cleavage. Recent multiplex strategies, namely two-tube, spatial separation one-tube, and homogeneo