A rapid visual detection method for indel polymorphisms in the bovine PRNP gene based on a duplex MIRA-LFD assay
Feng Guan
Insertion/deletion (indel) polymorphisms in the bovine PRNP gene promoter region (23 bp) and intron 1 (12 bp) influence gene expression and susceptibility to bovine spongiform encephalopathy (BSE). Conventional detection methods based on DNA sequencing are cumbersome and time-consuming. This study developed a visual detection method for these two polymorphic loci using multienzyme isothermal rapid amplification (MIRA) combined with lateral flow dipstick (LFD) technology, enabling efficient, low-