Various strains of Escherichia coli are significant foodborne pathogens that can threaten public health through contaminated food. In this study, a total of 217 animal-derived food products, including raw turkey meat, raw beef, fresh cheese, and raw milk, were collected from markets, butcher shops, dairies, and neighborhood markets in Istanbul during 2021, 2022, and 2023. E. coli isolates were detected in 13.4% of the samples using conventional microbiological methods, and identification was con