Coupling filter mating with nanopore selective sequencing expands capturable conjugative plasmid recovery in biological wastewater treatment systems

Nature Water, Published online: 01 September 2026; doi:10.1038/s44221-026-00695-w Filter mating coupled with nanopore selective sequencing recovers recipient-compatible conjugative plasmids from wastewater without plasmid extraction, revealing greater plasmid diversity and stage-specific profiles than extraction-based sequencing.