Inherent MS-cleavability of diazirine photo-cross-links enables residue-level structural analysis
Chun Tang
Nature Communications, Published online: 18 May 2026; doi:10.1038/s41467-026-73272-0 Photo-cross-linking is widely used in protein science, but site-specific identification remains elusive. Here, the authors show that alkyl diazirine cross-links are inherently MS-cleavable and develop an analytical framework that integrates seamlessly with existing search platforms.