Journal of Agricultural and Food Chemistry

Soybean peroxidase (SBP), a thermostable and pH-robust enzyme, holds promise as a biopolymer cross-linking agent. This study pioneers the secretory expression of SBP in <i>Saccharomyces cerevisiae</i>, a GRAS (generally recognized as safe) microbial host. To address expression bottlenecks, a synthetic hybrid promoter was designed through modular assembly, enabling stringent glucose-dependent repr…

The threat of antimicrobial resistance demands the exploration of underexplored microbial resources and innovative discovery methodologies. We introduce a strategy integrating predation-oriented strain prioritization with NMR-based metabolomics to accelerate antibiotic discovery from myxobacteria. By combining ecological prioritization, chromatographic prefractionation, universal NMR fingerprinti…

Stagonosporopsis cucurbitacearum is a main causal agent of gummy stem blight (GSB) in the melon field. Growers complain about the decreased control efficacy of DMIs for GSB in China recently. Difenoconazole (Dif) resistance frequencies reached 100% and 13.24% in <i>S. cucurbitacearum</i> isolates from Nanjing and Chongqing, respectively. Dif-sensitive isolates exhibited EC₅₀ values of …

Quinolizidine alkaloids (QAs) are toxic secondary metabolites in narrow-leafed lupin that defend against herbivores but constrain food and feed use. We developed a single-sample multiomics workflow for simultaneous extraction and quantitative analysis of QAs and proteins from individual leaves using urea/thiourea/CHAPS extraction with acetone precipitation, validated for QA quantification by LC-M…

<i>Alicyclobacillus acidoterrestris</i> is a type of contaminating bacteria that can cause the spoilage of juice. To develop a nonthermal control method, lysozyme-modified copper phosphate nanoflowers (Ly@Cu₃(PO₄)₂ NFs) were fabricated. The microstructure and composition of the hybrids were characterized, and the antibacterial performance was further evaluated. An…

Common wheat (<i>Triticum aestivum</i> L.) is a major food crop that is widely cultivated worldwide. Fusarium head blight (FHB) caused by <i>Fusarium graminearum</i> poses a growing threat to wheat yield and quality. Cysteine-rich secretory proteins (SCPs) mediate plant defense, but the role of wheat <i>TaSCPs</i> in FHB resistance remains unclear. Here, we report that TaSCP1-6B, a conserved, pos…

Nixtamalization is an alkaline cooking process known to lower aflatoxin B1 (AFB1) in maize, yet reported efficacy varies (28-100% reduction). Thus, existing data are not sufficient to inform nixtamalization practices in specific local contexts. To investigate this, we conducted a controlled lab study using AFB1-contaminated Guatemalan maize to evaluate the effect of current rural Guatemalan cooki…

Crocetin, a bioactive carotenoid derivative from saffron, exhibits antioxidant, anti-inflammatory, and metabolic regulatory properties. Its low natural abundance and limited extraction production hinder large-scale production. Microbial synthesis offers a promising alternative but is constrained by low carotenoid cleavage dioxygenase (CCD2) expression. Here, we engineered a zeaxanthin-producing <…

<i>Tuta absoluta</i> severely constrains global tomato production. We investigated the behavioral attraction of <i>Harmonia axyridis</i> to infested plants and the molecular basis of herbivore-induced plant volatile (HIPV) recognition. Y-tube bioassays showed statistically significant attraction of <i>H. axyridis</i> to the infested plant (χ² = 13.72, <i>P</i> < 0.05). Molecular dockin…

Alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA) are three major emerging <i>Alternaria</i> toxins in food. In this lncRNA-mRNA omics-based study, we first decrypt the blueprint: how emerging <i>Alternaria</i> toxins induce hepatic stellate cell LX-2 transdifferentiation for liver fibrosis. AOH and AME caused the fibrotic marker α-smooth muscle actin, extracellular…