ABSTRACT Rapid and sensitive diagnostic strategies are crucial for the prevention and control of tuberculosis (TB). Unlike the invasive TB diagnostic strategy in the clinic, here we introduce a convenient, portable, and non‐invasive system that is constructed by Ag@Au nanoflower (NF) array‐based sensing facemask and catalytic/plasmonic urchin‐shaped Au─Ag embedded covalent organic framework (U@COF) sensor. This system detects TB antigen ESAT‐6/CFP‐10 complex in droplet or sputum samples from a v